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BAX® System Real-Time PCR Assay for Genus Listeria

Utilization of a Real-Time PCR Listeria genus assay can reduce total time to product release and corrective action response. With a shortened, simplified sample preparation procedure and rapid real-time processing, this BAX® System assay provides a fast and accurate molecular testing method for Listeria species in food and environmental samples. With a faster time to result, reflexing positives onto the the BAX System RT L. mono assay provides a streamlined workflow to provide actionable results to customers.

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BAX Assay Kit2019 Genus Listeria

 


Benefits

  • Internal positive control automatically included in every reaction
  • Clear yes-or-no results icons eliminate the need for expert interpretation
  • LIMS-compatible for easy storage, sharing and printing
  • 100% inclusivity (including L. grayii) and 100% exclusivity in validation-level studies
  • Clear, reproducible results, independent of operator technique
  • Real-time processing and analysis complete for 96 samples in about 70 minutes
  • Each kit includes all required lysis reagents and pre-packaged PCR tablets
  • Validated with both reference method enrichment media and proprietary, single-step media for a faster time to result
  • Runs with the BAX® System real-time Salmonella and real-time L. monocytogenes assays to detect all three targets in a single batch

Specifications

  • Real-time
  • Target:   Listeria species
  • Enrichment time:   24-28 hours in 24 LEB Complete or 44-72 hours in standard media
  • PCR time:   about 70 minutes
  • Detection level:   104 cfu/mL, after enrichment

Catalog No.

Legacy Order Code  Hygiena Product Code Description Quantity
D15131113 KIT2019 BAX® System Real-Time Genus Listeria assay 96 tests per kit

Applications & Validations

 Validated Matrices for BAX® System Assays

The BAX® System holds the largest number of Validations and Approvals for PCR by third parties

 Click here to download this full list in PDF format

 

Listeria

 

Assay

Approved Matrices

Validation Reference

BAX® System Real‐Time PCR Assay for

Genus Listeria 

KIT2019 (Kit #D15131113)

 Frankfurters, cooked shrimp, spinach, bagged lettuce, queso fresco cheese, environmental surfaces AOAC PTM #081401 (Includes Actero™ Elite Enrichment Media from FoodChek™)
 All human food products and production environmental samples (excluding primary production samples)  AFNOR QUA 18/09-01/19(Validation study performed in accordance with EN ISO 16140-2)

Fit For Purpose Applications Data for BAX® Real-Time Listeria Assays

Product Category

Product

Assay

Dairy

AMF

Real-Time Genus Listeria

Block Cheese

Real-Time Genus Listeria

Cheese

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

X5 Genus Listeria

Coalho Cheese

Real-Time Genus Listeria

Lactose Powder

Real-Time Genus Listeria

Mozzerella Cheese

Real-Time Genus Listeria

WPC 34 - 24 LEB Comp

Real-Time Genus Listeria

WPC 34 - Demi Fraser/MOPS-BLEB

Real-Time Genus Listeria

Genus Listeria

WPC 80 - 24 LEB Comp

Real-Time Genus Listeria

WPC 80 - BLEB+Supplements

Real-Time Genus Listeria

Environmentals

Concrete 4" x 4" - Demi Fraser/MOPS-BLEB

Real-Time Genus Listeria

Environmental Surfaces Sponges and Swabs

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

X5 Genus Listeria

Plastic 4" x 4" - Insite L mono Glo Swab

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

X5 Genus Listeria

Plastic 4" x 4" - Sponge

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

X5 Genus Listeria

Plastic 4" x 4" - Sponge | 24 LEB Comp (2)

Real-Time Genus Listeria

Plastic 4" x 4" - Sponge | UVM/MOPS-BLEB (1)

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

X5 Genus Listeria

Plastic 4" x 4" - Sponge | UVM/MOPS-BLEB (2)

Real-Time Genus Listeria

Plastic 4" x 4" - World Bio Sponge | 24 LEB Comp

Real-Time Genus Listeria

Plastic4" x 4" - Sponge | 24 LEB Comp (1)

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

X5 Genus Listeria

Stainless Steel 4" x 4" - Sponge | UVM/MOPS-BLEB (2)

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

X5 Genus Listeria

Stainless Steel 4" x 4" - Demi Fraser/MOPS-BLEB

Real-Time Genus Listeria

Stainless Steel 4" x 4" - Sponge | 24 LEB Comp (1)

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

X5 Genus Listeria

Stainless Steel 4" x 4" - Sponge | 24 LEB Comp (2)

Real-Time Genus Listeria

Stainless Steel 4" x 4" - Sponge | UVM/MOPS-BLEB (1)

Real-Time Genus Listeria

Stainless Steel 4" x 4" - World Bio Sponge | 24 LEB Comp

Real-Time Genus Listeria

Fresh Fruit/Veg

Guacamole - 24 LEB Comp/MOPS-BLEB

Real-Time Genus Listeria

Genus Listeria

X5 Genus Listeria

Guacamole - BLEB+Supplements

Real-Time Genus Listeria

Genus Listeria

X5 Genus Listeria

Guacamole - Demi Fraser/MOPS-BLEB

Real-Time Genus Listeria

Genus Listeria

X5 Genus Listeria

Spinach and other vegetables

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

X5 Genus Listeria

Nuts

Pecans

Real-Time Genus Listeria

Genus Listeria

Genus Listeria 24E

Processed Fruit/Veg

Frozen Broccoli - 24 LEB Comp

Real-Time Genus Listeria

Genus Listeria

X5 Genus Listeria

Frozen Broccoli - BLEB+Supplements

Real-Time Genus Listeria

Genus Listeria

X5 Genus Listeria

Frozen Carrots - 24 LEB Comp

Real-Time Genus Listeria

Genus Listeria

Frozen Carrots - BLEB+Supplements

Real-Time Genus Listeria

Frozen Corn - 24 LEB Comp

Real-Time Genus Listeria

Genus Listeria

X5 Genus Listeria

Frozen Corn - BLEB+Supplements

Real-Time Genus Listeria

Genus Listeria

X5 Genus Listeria

Frozen Peas - 24 LEB Comp

Real-Time Genus Listeria

Genus Listeria

Frozen Peas - BLEB-Supplements

Real-Time Genus Listeria

RTE Meats

Frankfurters (RTE meat)

Real-Time Genus Listeria

Genus Listeria

X5 Genus Listeria

Seafood

Shrimp

Real-Time Genus Listeria

Genus Listeria 24E